

产品信息:
中(zhong)文名称:胃蛋白酶1:10000
英文名称:Pepsin 1:10000 Pepsin from porcine gastric mucosa
说明:用(yong)于制备F(ab′)2抗体片(pian)段,优(you)先(xian)水(shui)解C-末端的Phe, Leu 和 Glu,但是不水(shui)解Val, Ala 或Gly。
别名:Pepsin A;Pepsin from hog stomach
分(fen)子量:35 kDa
CAS:9001-75-6
外(wai)观(guan):类白色(se)粉(fen)末
特性:溶解性:溶于 0.01N HCl,参考(kao)浓度1 mg/ml。
酶活(huo):800 - 2500 U/mg蛋白
R:36/37/38-42 S:22-24-26-36/37
储存(cun)条件:2-8℃
说明: An enzyme in the gastric juice which hydrolyses proteins to give smaller polypeptides. Active only at acid pH, 1.5-2.5. Secreted as the inactive precursor pepsinogen, which is activated by acid. Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6.

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